
Process Analytics for Faster Biological & Cell Therapies
Cytomos® believes in affordable biopharmaceutical therapies serving the world's growing population made possible by intelligent bioprocessing
Intelligent bioprocessing requires novel, integrated and scalable process analytical technologies
Cytomos® Dielectric Spectroscopy (CDS™) is a full-stack, single-cell resolution analysis technology, spanning integrated sensors through to machine learning enhanced algorithms
CDSâ„¢ | The Dark Side of Spectroscopy

Different wavelengths of light refract at different angles
Different electric field frequencies behave similarly
CDSâ„¢ applies an electric field across single cells in suspension measuring 16,000+ frequencies per cell
Different interfaces in and around each cell refract different frequencies, generating a unique CDSâ„¢ spectrum per cell
A platform for evaluation & collaboration



Unbiased
Label-free spectroscopy
Predictive analysis potential
Scalable
Single-cell resolution of
cells flowing in suspension
Integrated
Microelectronic sensors can be implemented across production
Identifying cells without labels
Example: Identifying lymphocyte sub-populations within a mixture

(a) Scatterplot view after dimensional reduction

(b) CDSâ„¢ spectroscopic output
T-cell lymphoblasts (MOLT-4) identified from a 1:1 mixture with B-cell lymphoblasts (MM1.s) via different CDSâ„¢ spectra, confirmed with MM1.s only sample. Each dot in Figure (a) represents a cell identified from an individual CDSâ„¢ spectrum. Average spectrum for each gate shown in Figure (b). 12,144 cells measured in 15 mins.
Monitoring manufacturing drift
Example: Identifying subtle differences between T-cell lines

(a) Scatterplot view after dimensional reduction

(b) CDSâ„¢ spectroscopic output
T-cell lymphoblasts (MOLT-4 vs Jurkat) differentiated through their intrinsic CDSâ„¢ spectra. Each dot in Figure (a) represents a cell identified from an individual CDSâ„¢ spectrum. Average spectrum for each identified population shown in Figure (b). 4,103 cells measured in 15 mins.