Cytomos Limited

13 Melville Street

Edinburgh EH3 7PE

Scotland

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© Cytomos Limited 2019

Technology

Cytomos™ is developing a new class of label-free cytometer based upon our proprietary and patented cell analysis method 

Introducing: Cytomos™ Dielectric Spectroscopy (CDS)

Holistic single-cell resolution analysis

Cytomos™ Dielectric Spectroscopy (CDS) harnesses the intrinsic and distinctive dielectric properties of individual cells to perform rapid and label-free phenotyping.

CDS applies an electric field containing 1,000+ frequency components across individual cells in suspension as they flow across our proprietary microchip-integrated sensors.

A unique dielectric fingerprint is recorded for each cell. Our proprietary and powerful analysis algorithms extract information regarding cell shape, size, structure, viability and more while achieving sample throughput rates of  1,000 cells per second.

CDS uses dynamic electric fields to characterise the dielectric properties of matter. All matter, including cells, exhibit dielectric properties, meaning they can become polarised in an electric field. But what does that all mean?

Keep reading to find out more!

It's Spectroscopy... but not as we know it

Dielectric Spectroscopy - Key Concepts

Let's start with an analogy. The video shows how iron filings respond when exposed to a magnetic field generated by an electro-magnet.

When the magnet is off, no field is present, and the filings are randomly distributed. When the magnet is switched on, two phenomena occur: 

1. Polarisation

The magnetic field has induced a net positive and negative end within each filing. They have become polarised, illustrated by the compasses.

2. Orientation

The polarised compasses rotate to align themselves with the applied field. Larger compasses take longer to align than smaller compasses.

3. Relaxation

Finally, the filings return to their original state when the magnet is switched off and its magnetic field vanishes.   

Equivalent processes happen when matter is exposed to an electric field - which we apply to measure cells. 

Single-Cell Spectroscopy

Electric fields polarise matter inducing dipoles (equivalent to magnetic iron filings). Dipoles range in size from molecules to whole cells and take a finite time to align with an applied electric field. 

 

Large dipoles take longer to align than small dipoles, leading to a distinct frequency response for each and every dipole. CDS measures a broad frequency range to characterise a multitude of dipoles that provide a wealth of information about each cell.

Single-Cell Resolution

Single-cell resolution is achieved through our use of standard integrated microelectronics technology, enabling individual sensors to be manufactured at the same scale as individual cells.

Cellular Characteristics

Size and Morphology

Larger dipoles are measurable at lower frequencies corresponding to gross cellular differences. 

Membrane Integrity 

Intermediate frequencies access the smaller dipoles associated with the cellular membrane, permitting viability assessments and membrane dynamics to be measured.

Organelle Density

Higher frequencies access the smaller dipoles arising with in the cell interior, providing information upon organelle contents and density. 

Can CDS help you?

Have you an application that conventional analysis methods have failed to deliver results upon?

Get in touch with us to find out how Cytomos™ and CDS can help with your application.